Enzymes of the De novo Pyrimidine Biosynthetic Pathway in Leishmania tropica
نویسنده
چکیده
The pathway of de novo pyrimidine biosynthesis in the amastigote and Promastigote forms of Leishmania tropica has been investigated.Both parasite forms contained all six enzyme activities. Carbamoyl phosphate synthase (CPSase) utilizes Lglutamine and not ammonia as the amine group donor. Dihydroorotate dehydrogenase (DHO-DHase) was found to be cytoplasmic, where as orotate phosphoribosyltransferase (OPRTase) and orotidylate decarboxylase (ODCase) were found to be particulate. N(phosphonacetyl)-L-aspartate, dihydro-5azaorotate, 5-azaorotate and 6-azaUMP were found to be a potent inhibitor of the aspartate transcarbamoylase (ATCase), DHO-DHase, OPRTase, and ODCase, respectively.
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